Journal: Frontiers in Neuroanatomy

Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

doi: 10.3389/fnana.2023.1111008

Figure Lengend Snippet: Information on primary antibodies.

Article Snippet: Dopamine receptor D4 (D4R) , aa 176–185 of human D4R , Calbiochem; rabbit polyclonal; Cat# 324405, RRID:AB_564550 , 1:3,000.

Techniques: Purification

Journal: Frontiers in Neuroanatomy

Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

doi: 10.3389/fnana.2023.1111008

Figure Lengend Snippet: Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .

Article Snippet: Dopamine receptor D4 (D4R) , aa 176–185 of human D4R , Calbiochem; rabbit polyclonal; Cat# 324405, RRID:AB_564550 , 1:3,000.

Techniques: Immunohistochemistry, Immunohistochemical staining

Journal: Frontiers in Neuroanatomy

Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

doi: 10.3389/fnana.2023.1111008

Figure Lengend Snippet: D4R-immunoreactivity in astrocytes. (A,B) Moderate D4R-immunoreactivities of the cortical surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D4R (magenta) and GS (green) indicated strong D4R-immunoreactivities in pial astrocytes (arrowheads) in the prelimbic area. (E,F) Confocal imaging of double immunostaining for D4R (magenta) and GS (green) indicated strong D4R-immunoreactivities in pial astrocytes (arrowheads) in the primary motor area. (G,H) In layer I of the primary motor area, astrocytes showed strong immunoreactivities for D4R (arrows). (I,J) In layer V, weak D4R-immunoreactivities were observed in protoplasmic astrocytes (arrows). (K,L) In layer II of the somatosensory area, D4R-immunoreactivities were observed in GS-negative cells (asterisks). (M,N) In layer V of the primary motor area, D4R-immunoreactivities were observed in a pyramidal cell-like large cell (asterisks) and its process (arrows). (O–R) In layer I of the primary motor area, strong and granular immunoreactivities for D4R were observed (arrowheads), which were in close vicinity of the GS-positive structure (green), but not overlapped. Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 10 μm; (C,D,I–N) ; 5 μm (E–H) ; 2 μm (O–R) .

Article Snippet: Dopamine receptor D4 (D4R) , aa 176–185 of human D4R , Calbiochem; rabbit polyclonal; Cat# 324405, RRID:AB_564550 , 1:3,000.

Techniques: Imaging, Double Immunostaining, Staining